Convection:Article Title: Purification of astrocytes from transgenic rodents by fluorescence-activated cell sorting.
Article Snippet: .. Aspirator Bunsen burner and ethanol Conical tubes (50-mL) Dissection equipment: Decapitation scissors, large (ROBOZ RS-6820) Dissecting microscope Dissection scissors, curved (ROBOZ RS-5675) Forceps, #5 Forceps, curved Scalpel with No. 10 scalpel blade Scissors, medium-sized (ROBOZ RS-6702) Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) FACS machine Forceps Heat block preset to 34 ̊C in a sterile hood Hemacytometer Hood for sterile tissue culture Incubator preset to 37 ̊C and 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
Dissection:Article Title: Purification of astrocytes from transgenic rodents by fluorescence-activated cell sorting.
Article Snippet: .. Aspirator Bunsen burner and ethanol Conical tubes (50-mL) Dissection equipment: Decapitation scissors, large (ROBOZ RS-6820) Dissecting microscope Dissection scissors, curved (ROBOZ RS-5675) Forceps, #5 Forceps, curved Scalpel with No. 10 scalpel blade Scissors, medium-sized (ROBOZ RS-6702) Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) FACS machine Forceps Heat block preset to 34 ̊C in a sterile hood Hemacytometer Hood for sterile tissue culture Incubator preset to 37 ̊C and 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
Article Title: Purification of rat and mouse astrocytes by immunopanning.
Article Snippet: .. Aspirator Conical tubes (50 mL) Dissection equipment: • Decapitation scissors, large (ROBOZ RS-6820) • Dissecting microscope 422 Cite this article as Cold Spring Harb Protoc; 2013; doi:10.1101/pdb.prot074211 • Dissection scissors, curved (ROBOZ RS-5675) • Forceps, #5 • Forceps, curved • Scalpel with No.10 scalpel blade • Scissors, medium-sized (ROBOZ RS-6702) • Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) Heat block preset to 34 ̊C in a sterile hood Hemacytometer Incubator at 37 ̊C, 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
Microscopy:Article Title: Purification of astrocytes from transgenic rodents by fluorescence-activated cell sorting.
Article Snippet: .. Aspirator Bunsen burner and ethanol Conical tubes (50-mL) Dissection equipment: Decapitation scissors, large (ROBOZ RS-6820) Dissecting microscope Dissection scissors, curved (ROBOZ RS-5675) Forceps, #5 Forceps, curved Scalpel with No. 10 scalpel blade Scissors, medium-sized (ROBOZ RS-6702) Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) FACS machine Forceps Heat block preset to 34 ̊C in a sterile hood Hemacytometer Hood for sterile tissue culture Incubator preset to 37 ̊C and 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
Article Title: Purification of rat and mouse astrocytes by immunopanning.
Article Snippet: .. Aspirator Conical tubes (50 mL) Dissection equipment: • Decapitation scissors, large (ROBOZ RS-6820) • Dissecting microscope 422 Cite this article as Cold Spring Harb Protoc; 2013; doi:10.1101/pdb.prot074211 • Dissection scissors, curved (ROBOZ RS-5675) • Forceps, #5 • Forceps, curved • Scalpel with No.10 scalpel blade • Scissors, medium-sized (ROBOZ RS-6702) • Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) Heat block preset to 34 ̊C in a sterile hood Hemacytometer Incubator at 37 ̊C, 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
Sterility:Article Title: Purification of astrocytes from transgenic rodents by fluorescence-activated cell sorting.
Article Snippet: .. Aspirator Bunsen burner and ethanol Conical tubes (50-mL) Dissection equipment: Decapitation scissors, large (ROBOZ RS-6820) Dissecting microscope Dissection scissors, curved (ROBOZ RS-5675) Forceps, #5 Forceps, curved Scalpel with No. 10 scalpel blade Scissors, medium-sized (ROBOZ RS-6702) Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) FACS machine Forceps Heat block preset to 34 ̊C in a sterile hood Hemacytometer Hood for sterile tissue culture Incubator preset to 37 ̊C and 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
Article Title: Purification of rat and mouse astrocytes by immunopanning.
Article Snippet: .. Aspirator Conical tubes (50 mL) Dissection equipment: • Decapitation scissors, large (ROBOZ RS-6820) • Dissecting microscope 422 Cite this article as Cold Spring Harb Protoc; 2013; doi:10.1101/pdb.prot074211 • Dissection scissors, curved (ROBOZ RS-5675) • Forceps, #5 • Forceps, curved • Scalpel with No.10 scalpel blade • Scissors, medium-sized (ROBOZ RS-6702) • Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) Heat block preset to 34 ̊C in a sterile hood Hemacytometer Incubator at 37 ̊C, 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
FACS:Article Title: Purification of astrocytes from transgenic rodents by fluorescence-activated cell sorting.
Article Snippet: .. Aspirator Bunsen burner and ethanol Conical tubes (50-mL) Dissection equipment: Decapitation scissors, large (ROBOZ RS-6820) Dissecting microscope Dissection scissors, curved (ROBOZ RS-5675) Forceps, #5 Forceps, curved Scalpel with No. 10 scalpel blade Scissors, medium-sized (ROBOZ RS-6702) Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) FACS machine Forceps Heat block preset to 34 ̊C in a sterile hood Hemacytometer Hood for sterile tissue culture Incubator preset to 37 ̊C and 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
Blocking Assay:Article Title: Purification of astrocytes from transgenic rodents by fluorescence-activated cell sorting.
Article Snippet: .. Aspirator Bunsen burner and ethanol Conical tubes (50-mL) Dissection equipment: Decapitation scissors, large (ROBOZ RS-6820) Dissecting microscope Dissection scissors, curved (ROBOZ RS-5675) Forceps, #5 Forceps, curved Scalpel with No. 10 scalpel blade Scissors, medium-sized (ROBOZ RS-6702) Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) FACS machine Forceps Heat block preset to 34 ̊C in a sterile hood Hemacytometer Hood for sterile tissue culture Incubator preset to 37 ̊C and 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
Article Title: Purification of rat and mouse astrocytes by immunopanning.
Article Snippet: .. Aspirator Conical tubes (50 mL) Dissection equipment: • Decapitation scissors, large (ROBOZ RS-6820) • Dissecting microscope 422 Cite this article as Cold Spring Harb Protoc; 2013; doi:10.1101/pdb.prot074211 • Dissection scissors, curved (ROBOZ RS-5675) • Forceps, #5 • Forceps, curved • Scalpel with No.10 scalpel blade • Scissors, medium-sized (ROBOZ RS-6702) • Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) Heat block preset to 34 ̊C in a sterile hood Hemacytometer Incubator at 37 ̊C, 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
Hood:Article Title: Purification of astrocytes from transgenic rodents by fluorescence-activated cell sorting.
Article Snippet: .. Aspirator Bunsen burner and ethanol Conical tubes (50-mL) Dissection equipment: Decapitation scissors, large (ROBOZ RS-6820) Dissecting microscope Dissection scissors, curved (ROBOZ RS-5675) Forceps, #5 Forceps, curved Scalpel with No. 10 scalpel blade Scissors, medium-sized (ROBOZ RS-6702) Scoop, perforated (Moria MC17) Equilibration setup (a source of carbon dioxide [5% CO2/95% O2] with a line leading to a sterile hood) FACS machine Forceps Heat block preset to 34 ̊C in a sterile hood Hemacytometer Hood for sterile tissue culture Incubator preset to 37 ̊C and 10% CO2 Microscope, phase contrast Nitex mesh filter (Tetko HC3-20) Cut into 3-inch squares, wrap in small packets of foil, and autoclave. ..
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